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CPT1A Polyclonal antibody 119 Publications

Rabbit Polyclonal| Catalog number: 15184-1-AP

Featured Product KD/KO validated

濃度: 330 μg/ml

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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WB analysis using 15184-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WB analysis of A549 cells using 15184-1-AP

WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

WB analysis using 15184-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IF Staining of HepG2 using 15184-1-AP

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP experiment of HepG2 using 15184-1-AP

IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.

IHC staining of human liver cancer using 15184-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 10x lens).

IHC staining of human liver cancer using 15184-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 40x lens).

IF Staining of HepG2 using 15184-1-AP

Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

FC experiment of HeLa using 15184-1-AP

1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (15184-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, bovine, zebrafish
Positive WB detected in:
mouse liver tissue, A549 cells, HepG2 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver cancer tissue, mouse brain tissue, human kidney tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CPT1A fusion protein Ag7202
Full name:
carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight:
88 kDa
Observed molecular weight:
86 kDa
GenBank accession number:
BC000185
Gene ID (NCBI):
1374
Gene symbol
CPT1A
Synonyms
CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

Background

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.


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