EEF1B2 Antibody 10095-2-AP

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
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Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IHC staining of human pancreas cancer using 10095-2-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10095-2-AP (EEF1B2 antibody) at dilution of 1:50 (under 10x lens).

Immunofluorescent analysis of MCF-7 cells, using 10095-2-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

IP experiment of Jurkat using 10095-2-AP

IP Result of anti-EEF1B2 (IP:10095-2-AP, 4ug; Detection:10095-2-AP 1:500) with Jurkat cells lysate 2400ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

SKOV-3 cells, HEK-293 cells, PC-3 cells, HeLa cells, RAW264.7 , Jurkat cells

Positive IP detected in:

Jurkat cells

Positive IHC detected in:

human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

MCF-7 cells

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

IF : 1:10-1:100

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

EEF1B2 fusion protein Ag0135

Full name:

eukaryotic translation elongation factor 1 beta 2

Calculated molecular weight:

25 kDa

Observed molecular weight:

30-34 kDa

GenBank accession number:

BC000211

Gene ID (NCBI):

1933

Gene symbol

EEF1B2

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

Background

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.

Showing 1- [4] of [4] Publications

Author:		Mazan-Mamczarz Krystyna K
Pubmed ID:		16060370
Journal:		Biotechniques
Title:		En masse analysis of nascent translation using microarrays.
Application:		WB

Species:		human

Author:		Yuan Cao
Pubmed ID:		25436608
Journal:		PLoS One
Title:		Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2.
Application:		WB, IHC, IF
KD validated
Species:		mouse, human

Author:		Shuhei Sammaibashi
Pubmed ID:		30008712
Journal:		Front Microbiol
Title:		Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.
Application:		WB

Species:		human

Author:		Mazan-Mamczarz Krystyna K
Pubmed ID:		16537914
Journal:		Mol Cell Biol
Title:		Translational repression by RNA-binding protein TIAR.
Application:		WB,IF,IP

Species:		human

WB Figures

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Jurkat cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PC-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WB analysis of RAW264.7 using 10095-2-AP

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.