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NOB1 Polyclonal antibody 7 Publications

Rabbit Polyclonal| Catalog number: 10091-2-AP

Featured Product KD/KO validated

濃度: 300 μg/ml

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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WB analysis using 10091-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10091-2-AP (NOB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IHC staining of human lymphoma using 10091-2-AP

Immunohistochemical analysis of paraffin-embedded human lymphoma using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 10x lens).

IP experiment of HepG2 using 10091-2-AP

IP Result of anti-NOB1 (IP:10091-2-AP, 4ug; Detection:10091-2-AP 1:300) with HepG2 cells lysate 2400ug.

Species specificity:
human
Positive WB detected in:
HeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human lymphoma tissue, human placenta tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:10-1:100

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NOB1 fusion protein Ag0141
Full name:
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated molecular weight:
47 kDa
Observed molecular weight:
47-50 kDa
GenBank accession number:
BC000050
Gene ID (NCBI):
28987
Gene symbol
NOB1
Synonyms
ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1

Background

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.


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