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PCNA Monoclonal antibody 30 Publications

Mouse Monoclonal| Catalog number: 60097-1-Ig |CloneNo.: 10D10E11

濃度: 500 μg/ml

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

IHC staining of human gliomas using 60097-1-Ig

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of HepG2 using 60097-1-Ig

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60097-1-Ig (PCNA antibody), at dilution of 1:200 and CoraLite?488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB analysis of HEK-293 using 60097-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA Antibody) at various dilution incubated at room temperature for 1.5 hours.

WB analysis of HeLa using 60097-1-Ig

Western blot analysis of PCNA in various cell lines using Proteintech antibody 60097-1-Ig at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1.5 hours.

IP experiment of HepG2 using 60097-1-Ig

IP Result of anti-PCNA (IP:60097-1-Ig, 4ug; Detection:60097-1-Ig 1:300) with HepG2 cells lysate 3000ug.

FC experiment of MCF-7 using 60097-1-Ig

1X10^6 MCF-7 cells were stained with 0.2ug PCNA antibody (60097-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, pig, rat, rabbit, swine
Positive WB detected in:
HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:500-1:2000
IF : 1:250-1:1000

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PCNA fusion protein Ag7416
Full name:
proliferating cell nuclear antigen
Calculated molecular weight:
29 kDa/31 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC000491
Gene ID (NCBI):
5111
Gene symbol
PCNA
Synonyms
Cyclin, PCNA

Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa （PMID: 1358458）.


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