Untreated and IFN gamma treated A375 cells and A549 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HepG2, K-562, HSC-T6, and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WB result of PD-L1 antibody (66248-1-Ig, 1:1000 and 1:2000) with si-Control and si-PDL1 transfected HepG2 cells.

human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

pig lung tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Untreated and PNGase F-treated lysates of THP-1 cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:500 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:500 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 MDA-MB-231 cells were stained with 0.2 ug Anti-Human PD-L1/CD274 (66248-1-Ig, Clone: 2B11D11) and CoraLite?488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mosue IgG1 isotype control antibody and CoraLite?488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Samples were fixed with 90% MeOH.